Journal: bioRxiv
Article Title: Modeling viral and bacterial infections in human lung organotypic systems reveals strain specific host responses
doi: 10.1101/2025.03.31.644992
Figure Lengend Snippet: (A) IFNβ and (B) IFNλ3 proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
Article Snippet: For the IFNλ3 assay, the 21730-1 IgG1, kappa, mouse monoclonal antibody (PBL Assay Science) was used as a capture antibody after coating on paramagnetic beads (0.3 mg.mL -1 ), the MAB52591R IgG2a, mouse monoclonal antibody (R&D Systems, Minneapolis, MN, USA) was biotinylated (biotin/antibody ratio = 60/1) and used as the detector antibody at a concentration of 0.3 μg.mL -1 , and the 5259-IL-025/CF recombinant human IFNλ3 protein (R&D Systems) was used as calibrator.
Techniques: Infection